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  Development of a new and highly sensitive enzyme-linked
 immunosorbent assay system for human endothelial lipase.  

Endothelial lipase (EL) is a member of the lipoprotein lipase family. EL is primarily a phospholipase A1 which shows a high substrate specificity with high-density lipoprotein (HDL).  EL hydrolyzes phospholipids in HDL particles, and then promotes catabolism and remodeling of HDL particles. Thus, EL is a regulator of plasma levels of HDL-C in mice and in humans. One of the most significant characteristics of EL is that EL expression is highly increased by a variety of pro-inflammatory stimuli such as inflammatory cytokines, oxidative stress, angiotensin II, mechanical stress, etc, which may result in a reduction of plasma HDL-C levels. Inhibition of EL increases not only the plasma HDL-C level but also HDL particles preserved with anti-inflammatory actions in mice. However, the role of EL in HDL metabolism and atherosclerosis has not been fully elucidated in humans. Dr. Tatsuro Ishida in Cardiovascular Medicine and Immuno-Biological Laboratories, Inc., have established a new, highly sensitive sandwich ELISA to quantitatively measure human serum EL mass. This ELISA will be useful to clarify the impact of EL on HDL metabolism and its potential role in atherosclerosis in humans.

The detail of the new ELISA has been published in Clinical Chemistry.

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